Visual LAMP (loop-mediated isothermal amplification) field detection method for swine pasteurella

The invention discloses a visual LAMP (loop-mediated isothermal amplification) field detection method for swine pasteurella. The LAMP field detection method comprises the following steps: designing and synthesizing primers, constructing and identifying a recombinant plasmid standard substance, optim...

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Bibliographische Detailangaben
Hauptverfasser:	TIAN PEIPEI, TANG ZHAOXIN, CHEN YONGJIAN, ZHANG HUI, CAI QIUXIANG, LI YING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a visual LAMP (loop-mediated isothermal amplification) field detection method for swine pasteurella. The LAMP field detection method comprises the following steps: designing and synthesizing primers, constructing and identifying a recombinant plasmid standard substance, optimizing reaction conditions of LAMP, establishing an LAMP visual method, testing the sensitivity of the LAMP detection method, and testing the specificity of the LAMP detection method. The method has the beneficial effects that pasteurella nucleic acid carried in a sample can be quickly diagnosed through the RT-LAMP on-site detection method of the kit, detection equipment is simple, the operation is simple and convenient, the sensitivity is high, the specificity is strong, and the method is suitable for on-site diagnosis; compared with other molecular detection technologies, the loop-mediated isothermal amplification technology is high in sensitivity and simple and convenient to operate, the result can be directly in