Primer probe combination for detecting CAR (chimeric antigen receptor) cells and application of primer probe combination

The invention discloses a primer probe combination for detecting CAR (chimeric antigen receptor) cells and application of the primer probe combination. The nucleotide sequence of the primer comprises the sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 2; the nucleic acid sequence of the probe comprises a sequence as shown in SEQ ID NO. 3. Specific primers and probes are designed for CAR cells containing CD8 alpha transmembrane region-41 BB costimulatory molecules, a fluorescent quantitative PCR method for detecting the copy number of CAR genes is developed, then the CAR cells are analyzed, and the primer and probe combination is proved to have wide applicability through multi-angle verification analysis and limitation of detection standards. 本发明公开了一种检测CAR细胞的引物探针组合及其应用。所述引物的核酸序列包括SEQ ID NO.1-SEQ ID NO.2所示的序列；所述探针的核酸序列包括SEQ ID NO.3所示的序列。本发明针对含有CD8α跨膜区-41BB共刺激分子的CAR细胞，设计特定的引物和探针，开发检测CAR基因的拷贝数的荧光定量PCR方法，继而对CAR细胞进行分析，并通过多角度验证分析和检测标准的限定，证明本发明引物探针组合具有广泛的适用性。