Recombinant protease mutant, coding gene, vector, engineering bacterium and application in splitting (R, S)-2-ethyl tetrahydrofuroate
The invention discloses a recombinant protease mutant, a coding gene, a carrier, an engineering bacterium and application of the recombinant protease mutant to resolution of (R, S)-2-ethyl tetrahydrofuroate, and the application is as follows: taking supernatant crude enzyme liquid obtained by fermen...
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creator | LI YICHAO QIAN ZHAOXIA TONG MEIJUN ZHANG YINJUN WEI LITIAN |
description | The invention discloses a recombinant protease mutant, a coding gene, a carrier, an engineering bacterium and application of the recombinant protease mutant to resolution of (R, S)-2-ethyl tetrahydrofuroate, and the application is as follows: taking supernatant crude enzyme liquid obtained by fermentation culture of bacillus subtilis SCK6 or pure enzyme liquid obtained by purification of the crude enzyme liquid as a catalyst, taking racemic (R, S)-ethyl tetrahydrofuroate as a substrate, and carrying out enzymatic hydrolysis on the racemic (R, S)-2-ethyl tetrahydrofuroate to obtain the recombinant protease mutant, the coding gene, the carrier, the engineering bacterium and the resolution of the (R, S)-2-ethyl tetrahydrofuroate. According to the method, ethyl (S)-2-tetrahydrofuroate and (R)-2-tetrahydrofuroic acid are prepared through resolution under the conditions that the temperature is 10-60 DEG C and the speed is 500-1500 rpm by taking a phosphate buffer solution with the pH value being 8.0 as a reaction m |
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According to the method, ethyl (S)-2-tetrahydrofuroate and (R)-2-tetrahydrofuroic acid are prepared through resolution under the conditions that the temperature is 10-60 DEG C and the speed is 500-1500 rpm by taking a phosphate buffer solution with the pH value being 8.0 as a reaction m</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjjEOgkAQRWksjHqHsdMECtBELQ3RWFmgPRmXATaB2c3uYMIBvLdL4gGs_n_5r_jz6FOQMv1LM7KAdUYIPUE_SOAYlKk0N9AQUwxvUmJcDMSNZiI3LS9UEtrQA3IFaG2nFYo2DJrBBxKZtE0Rw2ObZAlJO3YgJA7bsXKmHpxBoWU0q7HztPrlIlpfL8_8lpA1JXmLKjyQMr-n6eGYpfvsdN7943wBlTtKnQ</recordid><startdate>20240405</startdate><enddate>20240405</enddate><creator>LI YICHAO</creator><creator>QIAN ZHAOXIA</creator><creator>TONG MEIJUN</creator><creator>ZHANG YINJUN</creator><creator>WEI LITIAN</creator><scope>EVB</scope></search><sort><creationdate>20240405</creationdate><title>Recombinant protease mutant, coding gene, vector, engineering bacterium and application in splitting (R, S)-2-ethyl tetrahydrofuroate</title><author>LI YICHAO ; QIAN ZHAOXIA ; TONG MEIJUN ; ZHANG YINJUN ; WEI LITIAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117821429A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LI YICHAO</creatorcontrib><creatorcontrib>QIAN ZHAOXIA</creatorcontrib><creatorcontrib>TONG MEIJUN</creatorcontrib><creatorcontrib>ZHANG YINJUN</creatorcontrib><creatorcontrib>WEI LITIAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LI YICHAO</au><au>QIAN ZHAOXIA</au><au>TONG MEIJUN</au><au>ZHANG YINJUN</au><au>WEI LITIAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Recombinant protease mutant, coding gene, vector, engineering bacterium and application in splitting (R, S)-2-ethyl tetrahydrofuroate</title><date>2024-04-05</date><risdate>2024</risdate><abstract>The invention discloses a recombinant protease mutant, a coding gene, a carrier, an engineering bacterium and application of the recombinant protease mutant to resolution of (R, S)-2-ethyl tetrahydrofuroate, and the application is as follows: taking supernatant crude enzyme liquid obtained by fermentation culture of bacillus subtilis SCK6 or pure enzyme liquid obtained by purification of the crude enzyme liquid as a catalyst, taking racemic (R, S)-ethyl tetrahydrofuroate as a substrate, and carrying out enzymatic hydrolysis on the racemic (R, S)-2-ethyl tetrahydrofuroate to obtain the recombinant protease mutant, the coding gene, the carrier, the engineering bacterium and the resolution of the (R, S)-2-ethyl tetrahydrofuroate. According to the method, ethyl (S)-2-tetrahydrofuroate and (R)-2-tetrahydrofuroic acid are prepared through resolution under the conditions that the temperature is 10-60 DEG C and the speed is 500-1500 rpm by taking a phosphate buffer solution with the pH value being 8.0 as a reaction m</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
title | Recombinant protease mutant, coding gene, vector, engineering bacterium and application in splitting (R, S)-2-ethyl tetrahydrofuroate |
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