Recombinant protease mutant, coding gene, vector, engineering bacterium and application in splitting (R, S)-2-ethyl tetrahydrofuroate

The invention discloses a recombinant protease mutant, a coding gene, a carrier, an engineering bacterium and application of the recombinant protease mutant to resolution of (R, S)-2-ethyl tetrahydrofuroate, and the application is as follows: taking supernatant crude enzyme liquid obtained by fermen...

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Hauptverfasser: LI YICHAO, QIAN ZHAOXIA, TONG MEIJUN, ZHANG YINJUN, WEI LITIAN
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QIAN ZHAOXIA
TONG MEIJUN
ZHANG YINJUN
WEI LITIAN
description The invention discloses a recombinant protease mutant, a coding gene, a carrier, an engineering bacterium and application of the recombinant protease mutant to resolution of (R, S)-2-ethyl tetrahydrofuroate, and the application is as follows: taking supernatant crude enzyme liquid obtained by fermentation culture of bacillus subtilis SCK6 or pure enzyme liquid obtained by purification of the crude enzyme liquid as a catalyst, taking racemic (R, S)-ethyl tetrahydrofuroate as a substrate, and carrying out enzymatic hydrolysis on the racemic (R, S)-2-ethyl tetrahydrofuroate to obtain the recombinant protease mutant, the coding gene, the carrier, the engineering bacterium and the resolution of the (R, S)-2-ethyl tetrahydrofuroate. According to the method, ethyl (S)-2-tetrahydrofuroate and (R)-2-tetrahydrofuroic acid are prepared through resolution under the conditions that the temperature is 10-60 DEG C and the speed is 500-1500 rpm by taking a phosphate buffer solution with the pH value being 8.0 as a reaction m
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Recombinant protease mutant, coding gene, vector, engineering bacterium and application in splitting (R, S)-2-ethyl tetrahydrofuroate
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