Recombinant glucose dehydrogenase as well as preparation method and application thereof

The invention provides recombinant glucose dehydrogenase as well as a preparation method and application thereof, and particularly, the recombinant glucose dehydrogenase is expressed by adopting genetic engineering escherichia coli, the recombinant glucose dehydrogenase is subjected to soluble expression in an escherichia coli system, and the expressed target protein is high in yield and has relatively high catalytic activity. Therefore, the method has the advantages of short production cycle, easy purification of expression products, low production cost and the like, and can realize industrial production of the recombinant glucose dehydrogenase. 本发明提供了一种重组葡萄糖脱氢酶及其制备方法和应用，具体地本发明采用了基因工程大肠杆菌表达重组葡萄糖脱氢酶，将重组葡萄糖脱氢酶在大肠杆菌系统进行可溶性表达，表达的目的蛋白产量高，并具备较高催化活性。因而具有生产周期短、表达产物容易纯化、生产成本低等优点，可实现重组葡萄糖脱氢酶的工业化生产。