Hybridoma cell secreting anti-pigeon IgG monoclonal antibody and application

The invention discloses a hybridoma cell capable of secreting an anti-pigeon IgG monoclonal antibody and application, the monoclonal antibody prepared by the invention is secreted by a hybridoma cell line 2H5, and the monoclonal antibody can specifically recognize pigeon immunoglobulin IgG as shown in a sequence SEQ ID NO: 6. The monoclonal antibody provided by the invention can be specifically combined with pigeon immune globulin IgG, is high in titer and good in affinity, can be used for specifically detecting the pigeon immune globulin IgG, and can be used for monitoring the antibody level in a pigeon body. 本发明公开了一种分泌抗鸽IgG单克隆抗体的杂交瘤细胞及应用，本发明制备的单克隆抗体由杂交瘤细胞系2H5所分泌，所述单克隆抗体能够特异性识别如序列SEQ ID NO：6所示鸽免疫球蛋白IgG。本发明提供的单克隆抗体能与鸽免疫球蛋白IgG特异结合，且效价高，亲和力好，能够用于特异检测鸽免疫球蛋白IgG，并用于鸽体内抗体水平监测。