Serum-free culture medium and culture method for hair follicle stem cells

The invention belongs to the technical field of cell culture, and particularly relates to a hair follicle stem cell serum-free culture medium and a culture method. The hair follicle stem cell serum-free medium provided by the invention comprises an MEM non-essential amino acid solution, an MEM vitamin solution, an L-glutamine solution, L-glutathione, sodium selenite, recombinant human serum albumin, recombinant human insulin, recombinant human transferrin, a recombinant human epidermal growth factor and a recombinant human basic fibroblast growth factor. The recombinant human platelet-derived growth factor-AB, recombinant human epithelial regulatory protein, recombinant human catalase, L-ascorbic acid, (+)-alpha-tocopheryl acetate, anethomycin, corticolipid ketone, L-carnitine, D-galactose, ethanolamine, linoleic acid, linolenic acid, progesterone, putrescine and a basal culture medium are clear in chemical components, free of exogenous exosome pollution, high in specificity, high in specificity and high in s