SILAC labeling method for mammalian cell line

The invention discloses a mammalian cell line SILAC labeling method, which relates to the field of biotechnology, and comprises the following steps: 1) digesting target cells cultured in a common culture medium with pancreatin, adding into an SILAC complete culture medium, neutralizing, centrifuging, removing supernatant to obtain cell precipitate, and adding SILAC into the cell precipitate to completely culture resuspended cells; (2) taking 1/3 of the total amount of the cells finally obtained in the step (1), paving the cells in a 25cm < 2 > culture bottle, supplementing a proper amount of SILAC complete culture medium, and culturing; (3) after the confluence degree of the cells in the step (2) reaches 90%-100%, digesting and discarding redundant cells, putting the remaining 10-20% of cells into a culture bottle, and supplementing the SILAC complete culture medium until the confluence degree of the cells reaches 90%-100% again; and step 4), repeating the step 3) for 6-8 times to complete SILAC labeling of t