Methanophilic genetically engineered bacterium of high-yield pullulan polysaccharide and construction method of methanophilic genetically engineered bacterium
The invention discloses a methanophilic genetically engineered bacterium for high yield of pullulan and a construction method of the methanophilic genetically engineered bacterium, and belongs to the technical field of industrial biology. The method comprises the following steps: introducing glucosy...
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| creator | FEI QIANG MEI JIAJIA FAN DAIDI XUE WENJIAO FU RONGZHAN |
| description | The invention discloses a methanophilic genetically engineered bacterium for high yield of pullulan and a construction method of the methanophilic genetically engineered bacterium, and belongs to the technical field of industrial biology. The method comprises the following steps: introducing glucosyltransferase UGT1 and pullulan synthase PUL1 into host bacteria, namely glycogen-producing methanophilic bacteria WONF2802, and carrying out overexpression on hexokinase HK1 and UDPG-pyrophosphorylase UDPGP1, so as to obtain the methanophilic genetically engineered bacteria PLA2 with high yield of pullulan polysaccharide. Under the condition that methane or methanol is used as a carbon source, the yield of the produced pullulan can reach 20 g/L or above, a new low-carbon biosynthesis way can be provided for commercialized production of the pullulan, and good industrial application prospects are achieved.
本发明公开了一种高产普鲁兰多糖的嗜甲烷基因工程菌及其构建方法，属于工业生物技术领域。通过在宿主菌产糖原嗜甲烷菌(Alkalicoccus glycogenes)WONF2802中导入葡萄糖基转移酶UGT1和普鲁兰合酶PUL1 |
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本发明公开了一种高产普鲁兰多糖的嗜甲烷基因工程菌及其构建方法，属于工业生物技术领域。通过在宿主菌产糖原嗜甲烷菌(Alkalicoccus glycogenes)WONF2802中导入葡萄糖基转移酶UGT1和普鲁兰合酶PUL1</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240405&DB=EPODOC&CC=CN&NR=117821346A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240405&DB=EPODOC&CC=CN&NR=117821346A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>FEI QIANG</creatorcontrib><creatorcontrib>MEI JIAJIA</creatorcontrib><creatorcontrib>FAN DAIDI</creatorcontrib><creatorcontrib>XUE WENJIAO</creatorcontrib><creatorcontrib>FU RONGZHAN</creatorcontrib><title>Methanophilic genetically engineered bacterium of high-yield pullulan polysaccharide and construction method of methanophilic genetically engineered bacterium</title><description>The invention discloses a methanophilic genetically engineered bacterium for high yield of pullulan and a construction method of the methanophilic genetically engineered bacterium, and belongs to the technical field of industrial biology. The method comprises the following steps: introducing glucosyltransferase UGT1 and pullulan synthase PUL1 into host bacteria, namely glycogen-producing methanophilic bacteria WONF2802, and carrying out overexpression on hexokinase HK1 and UDPG-pyrophosphorylase UDPGP1, so as to obtain the methanophilic genetically engineered bacteria PLA2 with high yield of pullulan polysaccharide. Under the condition that methane or methanol is used as a carbon source, the yield of the produced pullulan can reach 20 g/L or above, a new low-carbon biosynthesis way can be provided for commercialized production of the pullulan, and good industrial application prospects are achieved.
本发明公开了一种高产普鲁兰多糖的嗜甲烷基因工程菌及其构建方法，属于工业生物技术领域。通过在宿主菌产糖原嗜甲烷菌(Alkalicoccus glycogenes)WONF2802中导入葡萄糖基转移酶UGT1和普鲁兰合酶PUL1</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqVjTEOwjAMRbswIOAO5gAdShGwogrEAhM7MonbWHKdKEmHXoazQiUOANNb_vtvXryulB2qD46FDXSklNmgyAikHStRJAtPNJkiDz34Fhx3rhyZxEIYRAZBheBlTGiMw8iWANWC8ZpyHExmr9B_Kt5Odv9Xb1nMWpREqy8Xxfp8ujeXkoJ_UApopoNHc6uq_WFT1dvdsf5l8wa_71Z8</recordid><startdate>20240405</startdate><enddate>20240405</enddate><creator>FEI QIANG</creator><creator>MEI JIAJIA</creator><creator>FAN DAIDI</creator><creator>XUE WENJIAO</creator><creator>FU RONGZHAN</creator><scope>EVB</scope></search><sort><creationdate>20240405</creationdate><title>Methanophilic genetically engineered bacterium of high-yield pullulan polysaccharide and construction method of methanophilic genetically engineered bacterium</title><author>FEI QIANG ; MEI JIAJIA ; FAN DAIDI ; XUE WENJIAO ; FU RONGZHAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117821346A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>FEI QIANG</creatorcontrib><creatorcontrib>MEI JIAJIA</creatorcontrib><creatorcontrib>FAN DAIDI</creatorcontrib><creatorcontrib>XUE WENJIAO</creatorcontrib><creatorcontrib>FU RONGZHAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>FEI QIANG</au><au>MEI JIAJIA</au><au>FAN DAIDI</au><au>XUE WENJIAO</au><au>FU RONGZHAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Methanophilic genetically engineered bacterium of high-yield pullulan polysaccharide and construction method of methanophilic genetically engineered bacterium</title><date>2024-04-05</date><risdate>2024</risdate><abstract>The invention discloses a methanophilic genetically engineered bacterium for high yield of pullulan and a construction method of the methanophilic genetically engineered bacterium, and belongs to the technical field of industrial biology. The method comprises the following steps: introducing glucosyltransferase UGT1 and pullulan synthase PUL1 into host bacteria, namely glycogen-producing methanophilic bacteria WONF2802, and carrying out overexpression on hexokinase HK1 and UDPG-pyrophosphorylase UDPGP1, so as to obtain the methanophilic genetically engineered bacteria PLA2 with high yield of pullulan polysaccharide. Under the condition that methane or methanol is used as a carbon source, the yield of the produced pullulan can reach 20 g/L or above, a new low-carbon biosynthesis way can be provided for commercialized production of the pullulan, and good industrial application prospects are achieved.
本发明公开了一种高产普鲁兰多糖的嗜甲烷基因工程菌及其构建方法，属于工业生物技术领域。通过在宿主菌产糖原嗜甲烷菌(Alkalicoccus glycogenes)WONF2802中导入葡萄糖基转移酶UGT1和普鲁兰合酶PUL1</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Methanophilic genetically engineered bacterium of high-yield pullulan polysaccharide and construction method of methanophilic genetically engineered bacterium |
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