Methanophilic genetically engineered bacterium of high-yield pullulan polysaccharide and construction method of methanophilic genetically engineered bacterium

The invention discloses a methanophilic genetically engineered bacterium for high yield of pullulan and a construction method of the methanophilic genetically engineered bacterium, and belongs to the technical field of industrial biology. The method comprises the following steps: introducing glucosyltransferase UGT1 and pullulan synthase PUL1 into host bacteria, namely glycogen-producing methanophilic bacteria WONF2802, and carrying out overexpression on hexokinase HK1 and UDPG-pyrophosphorylase UDPGP1, so as to obtain the methanophilic genetically engineered bacteria PLA2 with high yield of pullulan polysaccharide. Under the condition that methane or methanol is used as a carbon source, the yield of the produced pullulan can reach 20 g/L or above, a new low-carbon biosynthesis way can be provided for commercialized production of the pullulan, and good industrial application prospects are achieved. 本发明公开了一种高产普鲁兰多糖的嗜甲烷基因工程菌及其构建方法，属于工业生物技术领域。通过在宿主菌产糖原嗜甲烷菌(Alkalicoccus glycogenes)WONF2802中导入葡萄糖基转移酶UGT1和普鲁兰合酶PUL1