Cell factor freeze-drying protective agent and application thereof, and preparation method of cell factor freeze-dried product

The invention provides a cell factor freeze-drying protective agent and application thereof, and a preparation method of a cell factor freeze-dried product, and relates to the technical field of biology. The cell factor freeze-drying protective agent comprises 0.5 to 1.5 wt% of bovine serum albumin, 0.3 to 1 wt% of proline, 0.03 to 0.08 wt% of polyvinylpyrrolidone, 1 to 1.5 wt% of trehalose, 0.2 to 1 wt% of glycerin, 0.5 to 1.5 wt% of sucrose and the balance of a buffer solution. The cell factor freeze-drying protective agent can improve the stability of a cell factor freeze-dried product so as to relieve the problem that the quality of cell factors is reduced after freeze-drying and redissolving in the prior art. 本发明提供了一种细胞因子冻干保护剂及其应用、细胞因子冻干品的制备方法，涉及生物技术领域。该细胞因子冻干保护剂包含0.5～1.5wt％牛血清白蛋白、0.3～1wt％脯氨酸、0.03～0.08wt％聚乙烯吡咯烷酮、1～1.5wt％海藻糖、0.2～1wt％甘油和0.5～1.5wt％蔗糖，余量为缓冲液。该细胞因子冻干保护剂能够提高细胞因子冻干品的稳定性，以缓解现有技术中细胞因子冻干复溶后质量下降等问题。