Preparation method of dilatanin

The invention relates to a preparation method of dilatanin. The preparation method comprises the following steps: step (1), forming a resin binding polypeptide with a peptide chain as shown in a formula (1); (2) carrying out cleavage reaction on the resin combined polypeptide and a peptide cutting reagent to separate the polypeptide from the resin to obtain a mixed solution; (3) removing the resin from the mixed solution to obtain a filtrate; and step (4): purifying the filtrate through high performance liquid chromatography. According to the method, diethyl ether is not needed, the production safety is high, and the operation process is simplified. # imgabs0 # 本发明涉及提供一种地非法林的制备方法，包括以下步骤：步骤(1)：形成具有式(1)所示肽链的树脂结合多肽；步骤(2)：将所述树脂结合多肽与切肽试剂进行裂解反应将多肽从树脂上分离，得到混合液；步骤(3)：从所述混合液中除去树脂，得到滤液；步骤(4)：通过高效液相色谱将所述滤液进行纯化。本发明的方法不需要使用乙醚，生产安全性高，并且精简了操作流程。#imgabs0#