Biotoxin detoxication agent as well as preparation method and application thereof

The invention provides a biotoxin detoxication agent and a preparation method and application thereof.The preparation method comprises the steps that magnetic Fe3O4 particles are subjected to functional modification through polydopamine, active groups are introduced to the surfaces of the magnetic F...

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Bibliographische Detailangaben
Hauptverfasser:	ZHAO SHIGUANG, CHU XINYING, LI WEI, LU YONGQI, YU JIANGJIE, CHEN SHENGXIAN, LIU QINGTAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSESC01D OR C01F CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES INORGANIC CHEMISTRY MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY PERFORMING OPERATIONS PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a biotoxin detoxication agent and a preparation method and application thereof.The preparation method comprises the steps that magnetic Fe3O4 particles are subjected to functional modification through polydopamine, active groups are introduced to the surfaces of the magnetic Fe3O4 particles, and a PDA-coated magnetic Fe3O4 nanometer material is obtained; lac and mediator Ms are co-immobilized by taking the laccase as a carrier, and the laccase/mediator co-immobilized detoxification agent which can be recycled and has biotoxin degradation capacity is obtained. According to the method disclosed by the invention, efficient degradation of DON is realized, and 94.42% of DON can be degraded within 60 minutes; after the catalyst is repeatedly used for 6 times, the degradation rate of DON can still reach 61.01%. By virtue of the detoxication agent, the living cells are increased from 47.08% to 62.43%, so that the cell apoptosis is obviously improved; the detoxification agent weakens the cytotox