Active fluorescence screening method of SARS-CoV-2 main protease Mpro

The invention discloses an active fluorescence high-throughput screening method for main protease Mpro of SARS-CoV-2. According to the method, polypeptide containing a fluorophore and a fluorescence quenching group (FRET effect) is used as a substrate, the FRET effect can enable the fluorescence effect of the substrate polypeptide to disappear, and the FRER effect is inhibited and the fluorescence intensity is increased under the action of cutting the substrate polypeptide by Mpro. The fluorescence intensity is associated with the enzyme activity of the main protease Mpro of the SARS-CoV-2, so that the method can be applied to screening of an Mpro inhibitor or activity detection of Mpro in a biological sample, has the characteristics of miniaturization, automation, rapidness, reliability and the like, is also suitable for high-throughput application, and can be used for detecting the activity of the Mpro in the biological sample. And the method can also be applied to development of a novel Mpro activity detec