Recombinant gene for improving expression quantity of E2 protein and application

The invention relates to the technical field of biological gene expression, in particular to a recombinant gene for improving the expression quantity of E2 protein and application. The recombinant gene is obtained by sequentially connecting a gene sequence of a signal peptide and a gene sequence of an E2 protein, and the gene sequence of the E2 protein can be efficiently expressed in a recombinant cell after codon optimization. Besides, a signal peptide sequence is introduced into the recombinant gene provided by the invention, and after the signal peptide sequence is connected with a codon-optimized E2 protein gene sequence, the expression quantity of E2 protein is remarkably improved. 本发明涉及生物基因表达技术领域，尤其涉及一种提高E2蛋白表达量的重组基因及应用。本发明所述重组基因由信号肽的基因序列与E2蛋白的基因序列顺次连接得到，其中，所述E2蛋白的基因序列经密码子优化，可在重组细胞中高效表达。另外，本发明提供的重组基因引入了信号肽序列，所述信号肽序列与密码子优化后的E2蛋白基因序列连接后，E2蛋白的表达量显著提升。