SsDNA nucleic acid aptamer for specifically recognizing grouper iridovirus and application of ssDNA nucleic acid aptamer

The invention provides an ssDNA (single-stranded deoxyribonucleic acid) aptamer for specifically recognizing grouper iridovirus. The nucleotide sequence of the ssDNA aptamer is shown as SEQ ID No: 1. The aptamer provided by the invention has relatively high affinity and specificity on grouper iridov...

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Hauptverfasser: WU MAN, YANG HUI, MOTO MASAYASU, ZHU LIBO, XU GUILIN, YU QING, SHI DEQIANG, QIU HENGTONG, LIU MINGZHU, LIU XIAONING, MOU RONGLI, LU LIULIN, LI PENGFEI, HUANG JING, KE KE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an ssDNA (single-stranded deoxyribonucleic acid) aptamer for specifically recognizing grouper iridovirus. The nucleotide sequence of the ssDNA aptamer is shown as SEQ ID No: 1. The aptamer provided by the invention has relatively high affinity and specificity on grouper iridovirus, and in addition, the aptamer also has the advantages of easiness in preparation and marking, no toxic or side effect on an organism, strong stability, easiness in storage and the like. When the aptamer provided by the invention is used for detecting the grouper iridovirus, the operation is simple, the consumed time is short, and the accuracy and the sensitivity are relatively high. Based on the aptamer, a kit or a molecular probe for specifically recognizing the grouper iridovirus can be constructed, so that rapid diagnosis of the grouper iridovirus can be realized, and the aptamer has a good application prospect in the aspect of green and healthy culture of groupers. 本发明提供一种特异性识别石斑鱼虹彩病毒的ssDNA核酸适体,其核苷酸序列如SEQ