Chimeric virus-like particle recombinant protein as well as preparation method and application thereof

The invention relates to a chimeric virus-like particle recombinant protein with an anti-tumor immune effect and a preparation method of the chimeric virus-like particle recombinant protein. The chimeric virus-like particle recombinant protein is formed by chimeric design of a hepatitis E virus capsid protein fragment (368-606aa) and an HPV16 early protein E7. Constructing a recombinant plasmid for expressing the recombinant protein, and obtaining escherichia coli recombinant expression bacteria through a cloning technology; and culturing the strain and carrying out induced expression, purification and renaturation to obtain the recombinant nano-protein particles. The chimeric virus-like particle recombinant protein disclosed by the invention has an obvious inhibition effect on tumors, can induce generation of strong humoral immunity and cellular immunity response, and also has 100% immune protection efficacy. 本申请涉及一种具有抗肿瘤免疫作用的嵌合病毒样颗粒重组蛋白及其制备方法，所述嵌合病毒样颗粒重组蛋白为戊型肝炎病毒衣壳蛋白片段(368-606aa)与HPV16型早期蛋白E7进行嵌合设计形成。构建表达重组