Specific antibody combined with canine red blood cells and application thereof

The invention discloses a specific antibody combined with canine red blood cells and application thereof. The specific antibody combined with the canine erythrocyte comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. According to the invention, dog red blood cell antigens are separated and extracted, immunized mice use a hybridoma cell technology to screen monoclonal antibodies with high sensitivity and high specificity, and the monoclonal antibodies can specifically bind to dog red blood cells of different dog types, do not bind to red blood cells of other species, and can be used as specific antibodies of test strips for identifying dog blood types. And the sample pad can also be used as a sample pad treating fluid component in a colloidal gold test strip for identifying a dog whole blood sample. The prepared dog