Method and apparatus for characterizing fluorescent particles by ratio

The present invention relates to a device and a method for characterizing fluorescently-labeled particles in a solution by analyzing changes in the fluorescence spectrum of the fluorescently-labeled particles. In particular, a sample of the fluorescently labeled particles is analyzed by fluorescence excitation under different conditions/environments, and the corresponding fluorescence emissions are measured. The particles are characterized by analyzing fluorescence emissions measured under different conditions/environments. More specifically, the present invention relates to methods and devices for characterizing intermolecular and/or intramolecular interactions, and/or conformational modification and/or localization of said fluorescently labeled particles by ratios. 本发明涉及用于通过分析荧光标记粒子的荧光光谱的变化来表征在溶液中的荧光标记粒子的设备和方法。特别是，在不同的条件/环境下通过荧光激发、并测量相应的荧光发射来分析所述荧光标记粒子的样品。通过分析在不同的条件/环境下测得的荧光发射来表征所述粒子。更具体而言，本发明涉及用于通过比率表征所述荧光标记粒子的分子间和/或分子内相互作用、和/或构象修饰和/或定位的方法和设备。