Compositions, methods and systems for single cell barcoding and sequencing

The present disclosure provides devices, methods, and systems for generating a plurality of droplets, generated from an aqueous mixture comprising a set of single cells and a set of functionalized particles configured for single cell assays, at a very high rate (e.g., at least one million droplets per minute, etc. Upon generation, the plurality of droplets can be stabilized in place within the region of the collection container, thereby providing a single tube workflow for single cell analysis. Further, the compositions implemented are structured to allow overloading of partitions with functionalized particles such that the partitioned single cells are co-localized with a subset of functionalized particles in a manner that allows discernible labeling and downstream analysis. 本公开提供了用于在收集容器内以极高的速率(例如，每分钟至少100万个液滴等)产生多个液滴的装置、方法和系统，多个液滴从包含单细胞集和被配置用于单细胞测定的功能化颗粒集的水性混合物产生。在产生后，多个液滴可以在收集容器的区域内稳定在适当位置中，从而为单细胞分析提供单管工作流程。此外，所实施的组合物被结构化以允许用功能化颗粒超载分区，使得分区的单细胞以允许可辨别的标记和下游分析的方式与功能化颗粒子集共定位。