Competitive ELISA kit for detecting African swine fever virus p30 protein antibody

The invention belongs to the technical field of biology, and relates to a competitive ELISA kit for detecting an African swine fever virus (ASFV) p30 protein antibody. The kit comprises an ELISA plate coated with ASFV p30 protein, an ASFV p30 protein monoclonal antibody marked by HRP (horse radish peroxidase), a positive serum control and a negative serum control. A heavy chain constant region of the ASFV p30 protein monoclonal antibody is of an IgM type, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is shown as SEQ ID NO: 3, and the amino acid sequence of a light chain variable region of the monoclonal antibody is shown as SEQ ID NO: 4. The kit provided by the invention has no cross reaction with positive serum of common swine disease related viruses, and the intra-batch and inter-batch repeated variable coefficient is less than 10%. The detection coincidence rate of the blocking ELISA kit and a foreign commercial kit is 96.69%, the Kappa value is 0.93, and the blocking