Method for on-line determination of nicotine metabolism and neurotransmitter effect in brain of living animal

The invention provides a method for on-line determination of nicotine metabolism and neurotransmitter effect in the brain of a living animal, and the method comprises the following steps: after the animal is exposed to nicotine, sampling by adopting a microdialysis technology, carrying out real-time derivatization on dialysate, directly feeding a derivatization product, carrying out ultra-high performance liquid chromatography on-line separation, and carrying out high resolution mass spectrometry to determine the nicotine metabolism and neurotransmitter effect in the brain of the living animal. The near real-time analysis of the nicotine metabolites and the classical neurotransmitters in the brain dialysate is realized. According to the method, integration of in-vivo minimally invasive sampling and online real-time analysis is realized, the problems of degradation, pollution and the like possibly caused in the storage and transfer processes of the sample are avoided, and the method has the advantages of simpl