Method for determining polyamine in breast milk and dairy products through gas chromatography-tandem mass spectrometry

The invention discloses a method for determining polyamine in breast milk and dairy products by gas chromatography-tandem mass spectrometry, which comprises the following steps: taking 20-200mu L of breast milk and dairy products, adding an isotope mixed standard solution, uniformly mixing, then adding a lysis solution, fully whirling, centrifuging, taking supernate, centrifugally concentrating and drying to obtain the polyamine in breast milk and dairy products. Re-suspending the dried sample in a mixed solution of ethyl acetate and trifluoroacetic anhydride, then derivatizing for 0.5-3 hours in a water bath at 40-80 DEG C, after derivatization is finished, continuing to perform centrifugal concentration and drying, re-dissolving dichloromethane, then performing solid-phase extraction and purification, blowing purified liquid nitrogen until the purified liquid nitrogen is nearly dry, and drying to obtain a sample; adding 20-200 [mu] L of a mixed solution of isopropanol and ethyl acetate, redissolving, and ca