Primer group for detecting HPA genotyping, kit and application

The invention provides a primer group and a kit for detecting HPA genotyping, the primer group comprises 35 groups, and the invention also provides a kit containing a chip of the primer group and an amplification reagent combination. The application has the following technical effects that the microfluidic chip is combined with a competitive allele specific amplification technology to carry out genetic typing qualitative detection on human platelet isoantigens (HPAs) HP1-35, compared with the existing detection technology, the kit can realize simultaneous detection of multiple sites, the volume of a PCR reaction system required by each site is only 1.15 mu L, the detection time is greatly shortened, the detection time is shortened, and the detection cost is reduced. The DNA template only needs 5-10 ng, the defects that an existing detection method is complex in operation, tedious in process and the like are overcome, and the method has wide application prospects and clinical reference value. 本发明提供了一种用于检测HPA基因