Method for promoting transfer of mercury from tobacco root to overground part

The invention provides a method for promoting transfer of mercury from tobacco roots to overground parts, which comprises the following steps: (1) extracting total RNA (Ribonucleic Acid) of tobacco leaves, carrying out reverse transcription to obtain cDNA (Complementary Deoxyribonucleic Acid), and carrying out PCR (Polymerase Chain Reaction); (2) carrying out BP clonase reaction by using the PCR product, and transforming an escherichia coli competent cell Top10, so as to obtain a plasmid pENTR-ABCCR; (3) carrying out LR (Local Repeat) clonase reaction by using the pENTR-ABCCR, and transforming the competent cell Top10 of the escherichia coli, so as to obtain a plasmid pSGRNAi-ABCCR; (4) the pSGRNAi-ABCCR is transformed into agrobacterium tumefaciens GV3101, and the transformed pSGRNAi-ABCCR And (5) cutting tobacco leaves into blocks, infecting and culturing, carrying out PCR (Polymerase Chain Reaction) on grown tobacco seedlings, and then carrying out agarose gel electrophoresis identification. The tobacco st