Strain for producing P34HB as well as construction method and application thereof

The invention discloses a strain for producing P34HB as well as a construction method and application of the strain. The construction method comprises the following steps: S1, amplifying a hadh-abfD-orfZ target gene sequence in vitro, and inserting the hadh-abfD-orfZ target gene sequence into a vector to obtain a vector plasmid; and S2, introducing the vector plasmid in the step S1 into the halomonas. The proportion of 4HB in P34HB synthesized by the engineering strain is high, and the engineering strain is expected to become a chassis strain for industrialization in the future. In the P34HB production process, the cytotoxicity can be reduced, and the P34HB content can be increased, so that the production cost is reduced, and industrial large-scale production is facilitated. 本发明公开了一种生产P34HB的菌株及其构建方法和应用。所述构建方法包括如下步骤：S1：体外扩增hadh-abfD-orfZ目的基因序列，插入载体中，得到载体质粒；S2：将S1所述的载体质粒导入盐单胞菌中。本发明的工程菌株合成P34HB中的4HB比例较高，有望成为未来产业化的底盘菌株。生产P34HB过程中，能够减低细胞毒性，提高P34HB含量，从而降低生产成本，有利于工业化大规模生产。