Rape gene BnaGH3 and application thereof in prevention and control of sclerotinia rot of colza

The invention belongs to the technical field of plant biology, and particularly provides a rape gene BnaGH3 and a regulation effect thereof in sclerotinia rot resistance of colza. The rape BnaGH3 gene is knocked out through a gene editing technology, a homozygous mutant of the BnaGH3 is obtained, and the positive regulation effect of the BnaGH3 gene on the sclerotinia sclerotiorum resistance is determined by inoculating sclerotinia sclerotiorum and detecting and analyzing the sclerotinia sclerotiorum resistance. Based on a research result of the invention, a transgenic vector of the gene can be constructed, and a new sclerotiniose-resistant rape material is created and obtained by adopting a genetic engineering technology. The invention has important significance and application value for prevention and treatment of sclerotinia rot of colza, and can be used in breeding of new varieties of colza. 本发明属于植物生物技术领域，具体的，本发明提供了油菜基因BnaGH3及其在油菜菌核病抗性中的调控作用。本发明通过基因编辑技术敲除油菜BnaGH3基因，获得了BnaGH3的纯合突变体，通过接种核盘菌，检测分析其对油菜菌核病的抗性，明