Multivalent aptamer cell sensor for sensitively detecting circulating tumor cells during epithelial-mesenchymal transition as well as preparation method and application of multivalent aptamer cell sensor

The invention discloses a multivalent aptamer cell sensor for sensitively detecting circulating tumor cells during epithelial-mesenchymal transition as well as a preparation method and application of the multivalent aptamer cell sensor. The sensor is prepared by connecting an aptamer nano-support formed by two nucleic acid aptamers through DNA self-assembly to the surface of a gold electrode, and CTCs in different EMT stages can be recognized, captured and released; the sensitivity is high, and the CTCs detection as low as 6 cells/mL is realized. The MAS-cell sensor provided by the invention can excite the design of other intelligent equipment, and can provide bright prospects for early diagnosis and advanced clinical analysis of cancers. 本发明公开了用于灵敏检测上皮-间质转化期间循环肿瘤细胞的多价适体细胞传感器及其制备方法和应用。所述的传感器由两种核酸适配体通过DNA自组装形成适配体纳米支架连接在金电极表面制成，可以实现对不同EMT阶段的CTCs进行识别、捕获和释放；灵敏度高，实现了低至6个细胞/mL的CTCs检测。本发明提出的MAS-细胞传感器将激发其它智能设备的设计，将为癌症的早期诊断和高级临床分析提供光明的前景。