Preparation method and application of salt-dissolving and growth-promoting compound microbial agent

The invention discloses a preparation method and application of a salt-dissolving and growth-promoting compound microbial inoculant, and the preparation method comprises the following steps: respectively activating and culturing escherichia coli BL-EF and Raoultella elongata SRPG-4, and adding IPTG...

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Bibliographische Detailangaben
Hauptverfasser:	LIU XIAOCHEN, SUN LINLIN, ZHOU XIAOHU, WU ZHANXING, TIAN FEI, HE YANHUI, JIA XINYU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED CULTURE MEDIA ENZYMOLOGY FERTILISERS FERTILISERS CHARACTERISED BY THEIR FORM FISHING FORESTRY HORTICULTURE HUMAN NECESSITIES HUNTING MANUFACTURE THEREOF METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENTSUBCLASSES OF CLASS C05 MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVINGA SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES,SOIL-CONDITIONERS, WETTING AGENTS MUTATION OR GENETIC ENGINEERING ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WATERING WINE
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Beschreibung
Zusammenfassung:	The invention discloses a preparation method and application of a salt-dissolving and growth-promoting compound microbial inoculant, and the preparation method comprises the following steps: respectively activating and culturing escherichia coli BL-EF and Raoultella elongata SRPG-4, and adding IPTG (isopropyl-beta-d-thiogalactoside) for low-temperature induction when the culture thalli of the escherichia coli BL-EF grow to the end of the logarithmic phase. The preparation method comprises the following steps: respectively diluting the obtained escherichia coli BL-EF fermentation liquor and Raoultella elongata SRPG-4 fermentation liquor for efficiently expressing ACC deaminase by using normal saline to prepare an escherichia coli BL-EF bacterial liquid and a Raoultella elongata SRPG-4 bacterial liquid for efficiently expressing ACC deaminase; and then mixing an escherichia coli BL-EF bacterial liquid and a Raoultella elongata SRPG-4 bacterial liquid which efficiently express ACC deaminase according to a volume