Fusion protein and application thereof in preparation of biosensing element

The invention provides a fusion protein, and belongs to the technical field of biological enzyme gene engineering and biosensing. The fusion protein comprises a carbohydrate binding module (CBM) and a lactate oxidase (LOD). The fusion protease of the lactate oxidase LOD and the carbohydrate binding module CBM is successfully constructed and prepared, and the fusion protease has good binding capacity with cellulose and can be prepared into a biological sensing element; and compared with an original LOD biosensor, the prepared biosensor is more sensitive to detection of a substrate, and the detection performance of an enzyme electrode is remarkably improved. 本发明提供了一种融合蛋白，属于生物酶基因工程和生物传感技术领域。所述融合蛋白包括碳水化合物结合模块CBM和乳酸氧化酶LOD。本申请成功构建并制备获得了一种乳酸氧化酶LOD和碳水化合物结合模块CBM的融合蛋白酶，该融合蛋白酶与纤维素具有很好的结合能力，能够制成生物传感元件，并且制得的生物传感器相较于原始LOD的生物传感器对底物的检测更为灵敏具有显著提高的酶电极的检测性能。