Sequencing method of sequence containing Poly structure

The invention provides a sequencing method of a sequence containing a Poly structure, and belongs to the technical field of gene therapy sequencing. The sequencing method comprises the following steps: determining a specific primer according to a target sequence; carrying out PCR amplification on the target sequence based on the specific primer; after a PCR amplification result is determined through gel electrophoresis, specific fragments are purified and intercepted through rubber tapping; sequencing the specific fragments based on a sequencing program to obtain sequencing data; wherein the unwinding time in the amplification cycle is 30 seconds. According to the sequencing method provided by the invention, a slippage phenomenon generated by sequencing is reduced by changing a sequencing amplification gradient amplification program under the condition that a target gene is not loaded into plasmids, so that the accuracy of a first-generation sequencing result is improved, and compared with an existing method,