Recombinant expression plasmid of short-chain dehydrogenase, recombinant genetically engineered bacterium and application thereof

The invention discloses a recombinant expression plasmid of short-chain dehydrogenase, a recombinant genetically engineered bacterium and application of the recombinant expression plasmid and the recombinant genetically engineered bacterium. The application refers to application of a wet cell obtained by induction culture of the recombinant genetically engineered bacterium or a short-chain dehydrogenase pure enzyme extracted by ultrasonication, separation and purification of the wet cell as a catalyst in preparation of alcohol compounds by asymmetric reduction of carbonyl compounds. The invention particularly relates to an application in preparation of (R)-4-chloro-3-hydroxybutyric acid ethyl ester by asymmetric reduction of 4-chloroacetoacetic acid ethyl ester. The catalyst has a wide substrate spectrum, can efficiently and highly selectively catalyze asymmetric reduction reactions of various pre-substrate ketones, and has excellent adaptability to different pH values and temperatures. 本发明公开一种短链脱氢酶的重组表达质粒、重组