Bensheng tobacco leaf callus induction and suspension cell culture method

The invention provides a Bensheng tobacco leaf callus induction and suspension cell culture method, and belongs to the technical field of plant tissue culture. The method comprises the following steps: inoculating Bensheng tobacco leaves into an induction culture medium for culturing to form calluses; and inoculating the callus into a suspension culture medium for culturing to obtain suspension cells. Compared with other methods for creating suspension cells from nicotiana benthamiana, the method disclosed by the invention has the characteristics of simplicity in operation and low maintenance cost. The nicotiana benthamiana suspension cells obtained by the invention have the characteristics of good dispersibility, rapid growth, chain-shaped cells and the like, and can be used for researches of phytophysiology, cytology and molecular biology. 本发明提供一种本生烟草叶片愈伤组织诱导及悬浮细胞培养方法，属于植株组织培养技术领域。本发明将本生烟草叶片接种至诱导培养基中培养，形成愈伤组织；将愈伤组织接种至悬浮培养基中培养，得悬浮细胞。本发明方法相比其他以本生烟草为来源的悬浮细胞创制方法，具有操作简单、维护成本低的特点。该发明获得的本生烟草悬浮细胞具有分散性良好、生长快速、细胞呈现链条