Affinity chromatography medium for purifying recombinant antibody and preparation method of affinity chromatography medium

The invention provides an affinity chromatography medium for recombinant antibody purification and a preparation method thereof.The affinity chromatography medium for recombinant antibody purification is characterized in that polyamide microspheres are used as a matrix, and the matrix is modified with glucan to obtain modified microspheres; carrying out epoxidation treatment on the modified microspheres to obtain epoxidized microspheres; and coupling a ligand with the epoxidized microspheres to obtain the affinity chromatography medium for purifying the recombinant antibody. The affinity chromatography medium for purifying the recombinant antibody is suitable for purifying the recombinant antibody, and is good in alkali resistance, stable in ligand and good in reuse effect. 本发明提供一种用于重组抗体纯化的亲和层析介质及其制备方法，所述的用于重组抗体纯化的亲和层析介质，以聚酰胺微球为基质，采用葡聚糖对所述基质进行改性，得到改性后的微球；将所述改性后的微球进行环氧化处理，得到环氧化微球；将配基与所述环氧化微球偶联，得到用于重组抗体纯化的亲和层析介质。本发明所述的用于重组抗体纯化的亲和层析介质，适用于重组抗体纯化，且耐碱性好、配体稳固、重复使用效果好。