Efficient sterile culture method for adult citrus branches

The invention discloses an efficient sterile culture method for adult citrus branches, which comprises the following steps: step 1, taking current-year semi-lignified branches with full bud points on the outer layer of the crown of a rock candy orange fruiting tree plant, and cutting the branches into segments with the length of 8-10cm to be used as explants of the adult citrus branches; 2, cleaning the explant, soaking the explant in ethanol under a sterile condition, soaking the explant in a NaClO solution, cutting the explant into stem segments with 1-2 bud points, inoculating the stem segments into an MS culture medium containing metasentine, and culturing until axillary buds grow out; 3, the stem tips of the axillary buds are grafted to the rootstock to form grafted seedlings, and the grafted seedlings are placed in an MS basic liquid culture medium to be cultured. According to the aseptic culture method for the adult citrus branches, aseptic treatment of the adult citrus branches can be effectively achi