Plasma protein binding rate determination method

The invention discloses a plasma protein binding rate determination method which comprises the following steps: firstly, directly transferring a certain volume of a plasma sample which is prepared at low temperature and contains a drug with test concentration to a volume exclusion chromatography for detection to obtain a chromatographic peak area Ap of the drug in the plasma sample at zero moment; then, directly transferring a certain volume of a blood plasma sample which is collected after in-vivo administration for a certain period of time or is incubated for a certain period of time at 37 DEG C in vitro and contains the medicine to the volume exclusion chromatography for detection, so as to obtain the chromatographic peak area Af of the medicine in the incubated blood plasma sample; and calculating the combination percentage of the medicine and the plasma protein by using a calculation formula. According to the characteristic that drug molecules and protein molecules are obviously different in size, the dr