Method for detecting microorganisms related to colorectal cancer tumor cells

The invention relates to a method for detecting colorectal cancer tumor cell related microorganisms, in particular to a method for eliminating intestinal fecal microbial contamination and detecting colorectal cancer tumor related microorganisms, and belongs to the technical field of biology. According to the method, intestinal tract wrinkles and villus structures are destroyed through rough shearing and enzymolysis, and pollution of fecal microorganisms in a sample structure is eliminated for the first time; filtering by using a 40-micron cell sieve to remove incompletely digested tissue blocks, further eliminating pollution of intestinal fecal bacteria, and collecting a single-cell suspension; and then repeatedly cleaning the collected single-cell suspension in combination with 300g of centrifugal force, removing intercellular or infirmly-combined passerby as much as possible, and retaining microorganisms in the cells and firmly colonized on the cells. And finally, serious pollution caused by huge and comple