Cultivation method for rapid rooting of hibiscus cannabinus

The invention provides a culture method for rapid rooting of hibiscus cannabinus, and belongs to the technical field of biology.The culture method comprises the steps that an HcNIR gene segment is connected to an overexpression vector, the obtained HcNIR gene overexpression vector is converted into...

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Hauptverfasser: DENG JIELOU, LI DEFANG, MAO XIAOTAO, LUAN MINGBAO, HE YUXU, CHENG GANG, DAI WEI, ZHENG JUNWEI, MAN BAIYING, ZHANG ZONGLIANG, LYU JIAQI, DENG YONG, CHEN YIFAN, WU YINGBAO, ZHANG GAOYANG, GU RUIZHE, ZHANG CHAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a culture method for rapid rooting of hibiscus cannabinus, and belongs to the technical field of biology.The culture method comprises the steps that an HcNIR gene segment is connected to an overexpression vector, the obtained HcNIR gene overexpression vector is converted into agrobacterium rhizogenes, and then agrobacterium rhizogenes liquid is prepared; after the hibiscus cannabinus seeds germinate, cutting cotyledons of the hibiscus cannabinus seeds to obtain explants; putting the explant in a culture medium with the wound facing downwards for pre-culture; after the pre-culture is finished, soaking the explant in the agrobacterium rhizogenes liquid, then taking out the explant, putting the explant in a recovery culture medium with a wound facing upwards, and carrying out dark culture; after the dark culture is finished, putting the explant with the wound facing downwards into a root induction culture medium to be cultured, so as to obtain the explant with a root system. According to t