Stage insert, microscope system, apparatus, method and computer program

A stage insert is provided. The stage insert (150) includes a first region (152) for receiving a sample holder (154). Further, the stage insert 150 comprises a second region 162 comprising a first calibration target 164 for calibrating a first parameter of the microscope system. The stage insert 150 further comprises a third region 172 comprising a second calibration target 174 for calibrating a second parameter of the microscope system. 提供载物台插入件。载物台插入件150包括用于容纳样本支持器154的第一区域152。此外，载物台插入件150包括第二区域162，第二区域162包括用于校准显微镜系统的第一参数的第一校准目标164。载物台插入件150还包括第三区域172，第三区域172包括用于校准显微镜系统的第二参数的第二校准目标174。