Preparation method of reagent for rapidly identifying muskmelon fungal wilt as well as product and application of reagent
The invention discloses a preparation method of a reagent for rapidly identifying muskmelon fungal wilt as well as a product and application of the reagent, and belongs to the technical field of prevention and treatment of plant fungal diseases. According to the technical scheme, the method comprise...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a preparation method of a reagent for rapidly identifying muskmelon fungal wilt as well as a product and application of the reagent, and belongs to the technical field of prevention and treatment of plant fungal diseases. According to the technical scheme, the method comprises the following steps: S1, preparing a muskmelon fungal fusarium wilt antigen; s2, preparing an antibody; s3, preparing a colloidal gold labeled monoclonal antibody; s4, preparing a treatment colloidal gold pad and preparing a sample pad; s5, preparing an NC membrane, and assembling double-antibody sandwich test paper; s6, cutting into a test strip. The detection method provided by the invention is convenient, rapid, specific and sensitive, can identify the muskmelon fungal fusarium wilt within a short time, does not need expensive instruments and equipment, has a high reference value for field real-time diagnosis and field disease samples, and is suitable for production, popularization and application.
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