Analysis method for measuring residual quantity of chiral pollutants in human digestive system

The invention belongs to the technical field of residual quantity determination of pollutants, and particularly relates to an analysis method for determining residual quantity of chiral pollutants in a human digestive system, which comprises the following steps: carrying out an in-vitro digestion simulation experiment on an aquatic product sample to obtain digestion samples at different digestion stages; and carrying out pretreatment on the digested samples at different stages, and determining the residual amount of HBCD by using a high performance liquid chromatography-tandem mass spectrometer after pretreatment. According to the method, shellfish which is one of dietary sources of aquatic products is taken as a research object, based on an in-vitro simulation experiment, the influence of an extraction method, a purification material and purification steps on the alpha-HBCD recovery rate is respectively investigated, and the method for determining the trace alpha-HBCD in the simulated digestive juice of the