Method for preparing human varicella immune globulin through full chromatography process

The invention relates to a method for preparing human varicella immune globulin by a full chromatography process. The method comprises the following steps: (1) screening and collecting raw material plasma; (2) preparing a human varicella immune globulin (VZV-IgG) stock solution by using a full chromatography process; the full chromatography process comprises the following steps: (1) adsorbing qualified raw material plasma by using A50, (2) carrying out affinity chromatography on the flow-through liquid obtained in the step (1), (3) precipitating the eluent obtained in the step (2) by using sodium caprylate, (4) carrying out DEAE ion exchange chromatography on the supernatant precipitated by using the sodium caprylate, and carrying out ultrafiltration on the obtained flow-through liquid to obtain a human varicella immune globulin (VZV-IgG) stock solution. 本发明涉及一种全层析工艺制备人水痘免疫球蛋白的方法；所述方法包括如下步骤：(1)筛选并收集原料血浆；(2)使用全层析工艺制备人水痘免疫球蛋白(VZV-IgG)原液；所述的全层析工艺包括如下步骤，1)合格的原料血浆使用A50吸附，2)步骤1)获得的流穿液使用亲和层析，3)步骤2)的洗脱液用辛酸钠沉淀，4)辛酸钠沉淀