Efficient boar semen freezing and unfreezing diluent as well as preparation method and application thereof
The invention relates to the technical field of livestock semen cryopreservation, in particular to an efficient boar semen freezing and unfreezing diluent as well as a preparation method and application thereof. The efficient boar semen freezing and unfreezing diluent is prepared from the following...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to the technical field of livestock semen cryopreservation, in particular to an efficient boar semen freezing and unfreezing diluent as well as a preparation method and application thereof. The efficient boar semen freezing and unfreezing diluent is prepared from the following raw materials: glucose, trisodium citrate, citric acid, disodium ethylene diamine tetraacetate, trihydroxymethyl aminomethane, penicillin and streptomycin. The invention further discloses a preparation method of the efficient boar semen freezing and unfreezing diluent and a fresh boar semen pre-diluent, a fresh boar semen resuspension diluent, an efficient boar semen freezing diluent and an efficient boar semen unfreezing diluent which are prepared from the efficient boar semen freezing and unfreezing diluent. The invention further discloses a freezing method and an unfreezing method of the boar semen. By means of the freezing and unfreezing method, the vitality of the frozen fresh pig sperms reaches 0.76 or above, |
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