Efficient boar semen freezing and unfreezing diluent as well as preparation method and application thereof

The invention relates to the technical field of livestock semen cryopreservation, in particular to an efficient boar semen freezing and unfreezing diluent as well as a preparation method and application thereof. The efficient boar semen freezing and unfreezing diluent is prepared from the following...

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Bibliographische Detailangaben
Hauptverfasser: DENG SHUANGYI, FENG KAI, GAO JIALIANG, CHOI BYEONGAN, WANG SHIYIN, GUO QINGHE, ZHANG WEI, LIU XIAONA, NING CHENGCHENG, CHO YOUNG-WOOK, YANG LIWEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of livestock semen cryopreservation, in particular to an efficient boar semen freezing and unfreezing diluent as well as a preparation method and application thereof. The efficient boar semen freezing and unfreezing diluent is prepared from the following raw materials: glucose, trisodium citrate, citric acid, disodium ethylene diamine tetraacetate, trihydroxymethyl aminomethane, penicillin and streptomycin. The invention further discloses a preparation method of the efficient boar semen freezing and unfreezing diluent and a fresh boar semen pre-diluent, a fresh boar semen resuspension diluent, an efficient boar semen freezing diluent and an efficient boar semen unfreezing diluent which are prepared from the efficient boar semen freezing and unfreezing diluent. The invention further discloses a freezing method and an unfreezing method of the boar semen. By means of the freezing and unfreezing method, the vitality of the frozen fresh pig sperms reaches 0.76 or above,