Evaluation method for in-vitro transdermal release of external composition containing azelaic acid

The invention discloses a method for evaluating in-vitro release of an azelaic acid external composition, which adopts a Franz diffusion cell device, takes a commercialized artificial membrane as simulated skin, takes a phosphate buffer solution as a receiving medium, and detects the content of azelaic acid in a receiving solution by using an ultra-high performance liquid chromatography-ultraviolet visible detector. And calculating the accumulated transdermal amount and the penetration rate of the azelaic acid. The in-vitro transdermal test method disclosed by the invention is simple, convenient, rapid, easy to operate and high in universality. 本发明公开了一种评价壬二酸外用组合物体外释放的方法，该方法采用Franz扩散池装置，以商品化的人工膜为模拟皮肤，以磷酸盐缓冲液为接收介质，用超高效液相色谱-紫外可见检测器检测接收液中壬二酸的含量，从而计算壬二酸累积透皮量和透过速率。本发明的体外透皮试验方法简便、快速、易操作、可普及性高。