Method and kit for detecting D-psicose

The invention belongs to the technical field of rapid detection, and discloses a method and a kit for detecting D-psicose. According to the method, whether the D-psicose is contained or not is judged according to color changes of a reaction product with known concentration and a reaction product of a sample to be detected, and the concentration of the D-psicose is determined according to a standard curve. According to the method disclosed by the invention, not only can the rapid detection of the D-psicose synthesized by an enzyme method or a biological fermentation method be realized, but also the D-psicose in natural products, foods and health-care products can be detected, and the high-throughput screening of the high-activity D-psicose-3-epimerase can also be realized. 本发明属于快速检测技术领域，公开了一种检测D-阿洛酮糖的方法及试剂盒。本发明所述方法通过已知浓度的反应产物和待测样品反应产物的颜色变化判断是否含有D-阿洛酮糖，并通过标准曲线确定D-阿洛酮糖的浓度。本发明所述方法不仅可以实现对酶法或生物发酵法合成的D-阿洛酮糖进行快速检测，而且可以对天然产物、食品、保健品中的D-阿洛酮糖进行检测，还可以对高活性的D-阿洛酮糖-3-差向异构酶进行高通量筛选。