Extraction and content determination method of total organic acid of Zhuang medicine tetrastigma planicaule

The invention discloses a method for extracting total organic acids from Zhuang medicine tetrastigma planicaule and determining the content of the total organic acids from the Zhuang medicine tetrastigma planicaule, and the method for extracting the total organic acids from the Zhuang medicine tetrastigma planicaule is obtained by optimizing an extraction process of the total organic acids from the tetrastigma planicaule by using a Box-Behnken response surface method and determining the content of the total organic acids from the tetrastigma planicaule by using an enzyme-labeled method. The extraction method comprises the following steps: crushing a tetrastigma planicaule medicinal material to obtain tetrastigma planicaule medicinal material powder, adding ethanol with the volume concentration of 45%-50% according to a solid-liquid ratio of 1g: 19-22mL, carrying out heating reflux extraction for 50-55 minutes to obtain a tetrastigma planicaule total organic acid extracting solution, determining the content by