Method for rapidly identifying transgenic soybean line and transformant component thereof by using nucleic acid dot hybridization technology

The invention relates to the field of genetic engineering. The invention discloses primers for rapidly identifying components of a transgenic soybean line and a transformant of the transgenic soybean line by using a nucleic acid dot blot hybridization method. The primers comprise an amplification pr...

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Bibliographische Detailangaben
Hauptverfasser:	TANG LIN, ZHENG RUFU, GE YUE, TANG YAMIAN, CHEN XIANXIAN, WANG FENGJUN, LIN JIEJIE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of genetic engineering. The invention discloses primers for rapidly identifying components of a transgenic soybean line and a transformant of the transgenic soybean line by using a nucleic acid dot blot hybridization method. The primers comprise an amplification primer of a soybean endogenous gene Lectin, an amplification primer of a transgenic soybean line DAS-68416-4, an amplification primer of a transgenic soybean line DAS-44406-6, an amplification primer of a transgenic soybean line DAS-81419, an amplification primer of a transgenic soybean line DAS-44406-6 and an amplification primer of a transgenic soybean line DAS-44406-6. The invention relates to an amplification primer of a transgenic soybean strain DP305423. The invention also provides a method for rapidly identifying the transgenic soybean line and the transformant component thereof by using a nucleic acid dot hybridization method. By adopting the method disclosed by the invention, high-throughput rapid detection