Application of PLCG2 and regulatory pathway related gene thereof in diagnosis and treatment of self-inflammatory diseases

The invention relates to the technical field of biological detection, in particular to application of PLCG2 and regulatory pathway related genes thereof in diagnosis and treatment of self-inflammatory diseases. The siRNA is designed and synthesized aiming at the nucleotide sequence of the PLCG2 gene, and after the siRNA is transferred into the human macrophage THP1, the expression of the PLCG2 gene can be efficiently inhibited, and the expression of various inflammatory factors of the THP1 cell can be remarkably reduced, so that the aim of relieving inflammatory symptoms is fulfilled. The PLCG2 siRNA molecule designed by the invention can be used for preparing medicines for treating various self-inflammatory diseases related to PLCG2 function enhancement, including IBD (Infectious Bursal Disease), and has a wide application prospect. 本发明涉及生物检测技术领域，具体涉及PLCG2及其调控通路相关基因在自身炎症性疾病诊断及治疗中的应用。本发明针对PLCG2基因的核苷酸序列设计合成siRNA，siRNA转入人巨噬细胞THP1后能高效抑制PLCG2基因的表达并显著降低THP1细胞多种炎症因子的表达，从而达到缓解炎性症状的目的。本发明设计的PLCG2 siRNA分子可用于制备治疗包括IBD在