Watermelon embryo gene delivery method
The invention discloses a watermelon embryo gene delivery method, which comprises the following steps: carrying out activation culture on recombinant agrobacterium tumefaciens containing a target gene to obtain a bacterial solution, and carrying out centrifugal collection, precipitation and resuspen...
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| creator | YAO XINLING JIANG YURONG |
| description | The invention discloses a watermelon embryo gene delivery method, which comprises the following steps: carrying out activation culture on recombinant agrobacterium tumefaciens containing a target gene to obtain a bacterial solution, and carrying out centrifugal collection, precipitation and resuspension to obtain a resuspension solution; the method comprises the following steps: accelerating germination of watermelon seeds to obtain embryo explants; rinsing the seed embryo explants, selecting the seed embryo explants with hypocotyls extending out of seed coats by 2-3mm, inoculating the resuspension at the positions 2-3mm away from the top ends of the hypocotyls, then placing the inoculated seed embryo explants in the resuspension, and performing oscillation treatment; burying the seed embryo explants in river sand for culturing, screening the seed embryo explants by adopting hygromycin, and then sowing and culturing the screened seed embryo explants; according to the delivery method, gene delivery can be comp |
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the method comprises the following steps: accelerating germination of watermelon seeds to obtain embryo explants; rinsing the seed embryo explants, selecting the seed embryo explants with hypocotyls extending out of seed coats by 2-3mm, inoculating the resuspension at the positions 2-3mm away from the top ends of the hypocotyls, then placing the inoculated seed embryo explants in the resuspension, and performing oscillation treatment; burying the seed embryo explants in river sand for culturing, screening the seed embryo explants by adopting hygromycin, and then sowing and culturing the screened seed embryo explants; according to the delivery method, gene delivery can be comp</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; 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the method comprises the following steps: accelerating germination of watermelon seeds to obtain embryo explants; rinsing the seed embryo explants, selecting the seed embryo explants with hypocotyls extending out of seed coats by 2-3mm, inoculating the resuspension at the positions 2-3mm away from the top ends of the hypocotyls, then placing the inoculated seed embryo explants in the resuspension, and performing oscillation treatment; burying the seed embryo explants in river sand for culturing, screening the seed embryo explants by adopting hygromycin, and then sowing and culturing the screened seed embryo explants; according to the delivery method, gene delivery can be comp</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFALTyxJLcpNzcnPU0jNTSqqzFdIT81LVUhJzcksSy2qVMhNLcnIT-FhYE1LzClO5YXS3AyKbq4hzh66qQX58anFBYnJQE0l8c5-hobm5gaGZkYWjsbEqAEAhignvw</recordid><startdate>20240315</startdate><enddate>20240315</enddate><creator>YAO XINLING</creator><creator>JIANG YURONG</creator><scope>EVB</scope></search><sort><creationdate>20240315</creationdate><title>Watermelon embryo gene delivery method</title><author>YAO XINLING ; JIANG YURONG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN117701628A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YAO XINLING</creatorcontrib><creatorcontrib>JIANG YURONG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YAO XINLING</au><au>JIANG YURONG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Watermelon embryo gene delivery method</title><date>2024-03-15</date><risdate>2024</risdate><abstract>The invention discloses a watermelon embryo gene delivery method, which comprises the following steps: carrying out activation culture on recombinant agrobacterium tumefaciens containing a target gene to obtain a bacterial solution, and carrying out centrifugal collection, precipitation and resuspension to obtain a resuspension solution; the method comprises the following steps: accelerating germination of watermelon seeds to obtain embryo explants; rinsing the seed embryo explants, selecting the seed embryo explants with hypocotyls extending out of seed coats by 2-3mm, inoculating the resuspension at the positions 2-3mm away from the top ends of the hypocotyls, then placing the inoculated seed embryo explants in the resuspension, and performing oscillation treatment; burying the seed embryo explants in river sand for culturing, screening the seed embryo explants by adopting hygromycin, and then sowing and culturing the screened seed embryo explants; according to the delivery method, gene delivery can be comp</abstract><oa>free_for_read</oa></addata></record> |
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| language | chi ; eng |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Watermelon embryo gene delivery method |
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