Watermelon embryo gene delivery method

The invention discloses a watermelon embryo gene delivery method, which comprises the following steps: carrying out activation culture on recombinant agrobacterium tumefaciens containing a target gene to obtain a bacterial solution, and carrying out centrifugal collection, precipitation and resuspen...

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Bibliographische Detailangaben
Hauptverfasser: YAO XINLING, JIANG YURONG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a watermelon embryo gene delivery method, which comprises the following steps: carrying out activation culture on recombinant agrobacterium tumefaciens containing a target gene to obtain a bacterial solution, and carrying out centrifugal collection, precipitation and resuspension to obtain a resuspension solution; the method comprises the following steps: accelerating germination of watermelon seeds to obtain embryo explants; rinsing the seed embryo explants, selecting the seed embryo explants with hypocotyls extending out of seed coats by 2-3mm, inoculating the resuspension at the positions 2-3mm away from the top ends of the hypocotyls, then placing the inoculated seed embryo explants in the resuspension, and performing oscillation treatment; burying the seed embryo explants in river sand for culturing, screening the seed embryo explants by adopting hygromycin, and then sowing and culturing the screened seed embryo explants; according to the delivery method, gene delivery can be comp