RDGAWKY gene targeted medicament for preventing and treating grain storage pest beetle and application thereof
The invention provides an RDGAWKY gene targeting agent for preventing and controlling grain storage insect grain beetles and application of the RDGAWKY gene targeting agent, and belongs to the technical field of grain storage insect prevention and control. The RDGAWKY gene targeting agent for preven...
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| creator | XU HONGLEI TANG PEI'AN WU XUEYOU |
| description | The invention provides an RDGAWKY gene targeting agent for preventing and controlling grain storage insect grain beetles and application of the RDGAWKY gene targeting agent, and belongs to the technical field of grain storage insect prevention and control. The RDGAWKY gene targeting agent for preventing and treating grain storage pest beetle is prepared by adopting a method comprising the following steps: carrying out reverse transcription on total RNA (Ribonucleic Acid) of the beetle into cDNA (Complementary Deoxyribose Nucleic Acid), carrying out PCR (Polymerase Chain Reaction) amplification by taking the cDNA as a template and adopting a primer pair as shown in SEQ ID No.1 and SEQ ID No.2, and carrying out in-vitro transcription on a PCR amplification product to synthesize dsRNA, so as to obtain the RDGAWKY gene targeting agent. After the RDGAWKY gene targeted medicament for preventing and treating the grain storage pest beetles is applied, the grain storage pest beetles can be effectively prevented and tr |
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The RDGAWKY gene targeting agent for preventing and treating grain storage pest beetle is prepared by adopting a method comprising the following steps: carrying out reverse transcription on total RNA (Ribonucleic Acid) of the beetle into cDNA (Complementary Deoxyribose Nucleic Acid), carrying out PCR (Polymerase Chain Reaction) amplification by taking the cDNA as a template and adopting a primer pair as shown in SEQ ID No.1 and SEQ ID No.2, and carrying out in-vitro transcription on a PCR amplification product to synthesize dsRNA, so as to obtain the RDGAWKY gene targeting agent. 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The RDGAWKY gene targeting agent for preventing and treating grain storage pest beetle is prepared by adopting a method comprising the following steps: carrying out reverse transcription on total RNA (Ribonucleic Acid) of the beetle into cDNA (Complementary Deoxyribose Nucleic Acid), carrying out PCR (Polymerase Chain Reaction) amplification by taking the cDNA as a template and adopting a primer pair as shown in SEQ ID No.1 and SEQ ID No.2, and carrying out in-vitro transcription on a PCR amplification product to synthesize dsRNA, so as to obtain the RDGAWKY gene targeting agent. 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The RDGAWKY gene targeting agent for preventing and treating grain storage pest beetle is prepared by adopting a method comprising the following steps: carrying out reverse transcription on total RNA (Ribonucleic Acid) of the beetle into cDNA (Complementary Deoxyribose Nucleic Acid), carrying out PCR (Polymerase Chain Reaction) amplification by taking the cDNA as a template and adopting a primer pair as shown in SEQ ID No.1 and SEQ ID No.2, and carrying out in-vitro transcription on a PCR amplification product to synthesize dsRNA, so as to obtain the RDGAWKY gene targeting agent. After the RDGAWKY gene targeted medicament for preventing and treating the grain storage pest beetles is applied, the grain storage pest beetles can be effectively prevented and tr</abstract><oa>free_for_read</oa></addata></record> |
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| language | chi ; eng |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | RDGAWKY gene targeted medicament for preventing and treating grain storage pest beetle and application thereof |
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